Observation of zebrafish embryonic and larval development at different doses of curcumin solution: the disparity between safe and toxic

The aim of the study is to perform a fast and efficient toxicity test that demonstrates safety or levels in embryonic development zebrafish exposed different doses curcumin. Embryotoxicity tests were performed according OECD guideline. A standard dose 100 mg/kg curcumin per embryo weight was determined. stock solution dissolved dimethylsulfoxide then used serial dilution dechlorinated water identify safe concentration. at concentrations 0.6; 1.25; 2.50; 5; 6.25; 10; 12.5; 25; 50; μM/mL, together with control containing only water. After start exposure, embryos monitored an optical microscope 8, 24, 48, 72, 96 hours post-exposure possible changes mortality. We observed higher influence appearance teratogenic characteristics such as eye malformation, retarded growth, yolk edema, crooked syrup, short syrup. results also analyzed from survival rate used, concluding concentrations, there significant difference survival.